Progress of granzyme-induced apoptosis in tumor cells / 肿瘤研究与临床

Granzyme exists in the cytotoxic granules and belongs to a highly homologous serine protease family. Granzyme is synthesized and released from natural killer cells and cytotoxic lymphocytes. It induces multiple programmed death with the participation of perforin and is an important medium for immune cells to exert anti-tumor effects. This article reviews the granzyme-mediated tumor cell death and the relationship between granzyme and kinds of ways of cell apoptosis, in order to provide new theoretical basis and therapeutic strategies for tumor therapy.

Porcine transcription factor AP-2δ promotes porcine circovirus type 2 replication through enhancing the activity of the rep gene promoter / 生物工程学报

Several putative transcription factor binding sites (TFBSs) exist in the PCV2 rep gene promoter. To explore if porcine circovirus type 2 (PCV2) could regulate the viral replication by using these TFBSs, we conducted electrophoretic mobility shift assay (EMSA), DNA-pull down and liquid chromatography-tandem mass spectrometric (LC-MS/MS) assays. EMSA confirmed the binding activity of the rep gene promoter with nuclear proteins of host cells. DNA-pull down and LC-MS/MS identified the porcine transcription factor AP-2δ (poTFAP2δ) could bind the PCV2 rep gene promoter. Dual-luciferase reporter assay, quantitative real-time PCR, Western blotting and indirect immunofluorescent assay demonstrated that poTFAP2δ could not only promote the activity of the rep gene promoter, but also enhance the transcription/translation activity of the rep/cap gene and the virus titer of PCV2 during the entire life cycle of PCV2 infection. This study revealed the molecular mechanism of PCV2 using host proteins to enhance the viral replication, provided a new perspective for studying the pathogenic mechanism of PCV2 from virus and host interactions, and provided a theoretical basis for developing highly effective PCV2 vaccines.

Characteristics of childhood traumatic experiences in borderline personality disorder in college students / 中国心理卫生杂志

Objective: To examine the characteristics of childhood traumatic experiences in borderline personality disorder (BPD) in college students. Methods: The Personality Diagnosis Questionnaire-~(4+) (PDQ-~(4+)), Personality Disorder Interview- Ⅳ (PDI - Ⅳ) and Childhood Trauma Questionnaire -28 Short Form (CTQ-SF) were administered to 3227 college students of sophomore and junior. Results: (1) In the investigation of PDQ-~(4+), 31 subjects with childhood traumatic experiences (0.96%) were diagnosed as BPD, and the total score of BDL sub-scale was (2. 62 ± 1.70), including 18 females and 13 males. In CTQ test, the scores of bad environment in females were significantly higher than that in males [(13.63±4.54) vs. (9.83±1.95), P<0.01] . (2) Subjects with BPD got higher scores than normal controls in CTQ-SF, such as the emotional abuse [(2.11 ±0.77) vs. (1.66±0.49), P<0.01] .Conclusion: college students with borderline personality disorder mostly have d different childhood traumatic experiences, and there exists a sex difference.